Why is my monotub taking so long to colonize. Small/Premature Mushrooms- many factors involved here, from lack of FAE, lack of humidity, off moisture content, or simply bad genetics. Aug 22, 2023 · Taping the holes. My first attempt at a monotub is working perfectly so far, Penis Envy LC>Rye>HPoo/straw. Initiate Fruiting: If fully colonized after 10 days, prepare for fruiting. IME mushrooms don't like being babied. Precautions: Ensure that the calcium chloride is kept out of direct contact with the substrate or mushrooms, as direct exposure can harm your culture. Remember to fan twice per day until the substrate is no longer producing mushrooms. Throughout the day mist the inside of the plastic bag so visible droplets of water are present. Sep 4, 2021 · The plastic bag will serve as your fruiting chamber. Why is this taking so long to colonize? Is the substrate too rich in nutrients? My other monotub with the enoki is doing really well, why dont the buttom Don't know what these people are expecting, but that's pretty covered in mycelium. I put my duct tape on the bottom holes of the mono and use it to hold the liner in place while working. Using less grain in the ratio will still produce perfectly fine results. This is a follow up to this post showing the results of my first flush of my first time growing mushrooms. So here it is, im trying my first monotub and took a little risk doing a 1:4 spawn ratio, however so far so good. I spawned 18 cakes to 20 pounds of HMC (About 18 quarts). Its been about 10 days, and it looks like the substrate is fully colonized, with happy rhizos reaching out to say HELLO! I'm roughly following Fahtsters monotub tek. And a temp prob controlling the heat. Like the other user said, I'd aim for around six jars Aug 10, 2021 · 13:33. So with 10 days of fruiting conditions on an uncased monotub 🔴 Monotub is about "putting all the eggs in one basket". Another trick is to mist the sides and top of your monotub (and eventually dubtub). Even in total darkness, the mushrooms that form will grow straight up, opposite gravity. This is my first time growing with monotubs and they seem to have stalled. comments sorted by Best Top New Controversial Q&A Add a Comment Oct 6, 2012 · So you have some sub that isn't colonized you see, fruit it and get that part that is colonized to fruit. Im planning on trying a late casing, casing at the Cultivation. Smaller Monotubs should use higher holes so they can use more substrate). A monotub is a simple and effective way to grow mushrooms, such as Lion’s Mane. May 24, 2023 · Identifying Contamination. Step 1 : Mark Air Vent Hole Positions on the Tub. Shit happened on my first grow with brf, half colonized, fruited, got fruits. Guide 1 – Using a Drill to create a Monotub. They look like huge Tupperware, essentially. Place your container/block so there will be room for your mushrooms to grow inside the tub without hitting the sides. Your casing is not completely colonized so the major pinning triggers (surface moisture evaporating and higher oxygen levels) are not present where the myc currently is. Exactly this. #15724523 - 01/27/12 10:46 AM (12 years, 2 days ago) Edit. the first is z strain and it was put into the tub 14 days ago. Apr 26, 2022 · Its been about 13 days since the bulk sub appeared fully colonized. Just make sure to not open them until all of the grain is 100% colonized. Don't forget that even if it looks fully colonized, it still takes awhile for the inside to colonize too. However, this doesn’t mean that the mycelium needs any oxygen. Other than that just leave the lid on till fully colonized. Quote. The grain jars i used took about 3 months to fully colonize and at the end they Apr 5, 2021 · STEP SEVEN – The “Spawn Run”. Jul 31, 2014 · The holes should remain covered until 100% colonized and/or ready to fruit. How long do these normally take to colonize? I spawned the popcorn seed to coir on 12/30/2015. One of the most frequent hurdles in monotub cultivation is contamination. This part of the tek is based on Monotub Tek (Ironed polyfil filters for bottom holes) and My Magnetic SAB Arm-hole Covers. Nutrients need to be taken before the tub goes into fruiting. Substrate Preparation: The first step is to prepare the substrate, which will serve as the growing medium for the mushrooms. Aug 19, 2020 · Monotub not colonized after 2 weeks! Please Help! Hello everyone, let me preface my question by stating that I am a newb, and this is my first attempt at a Monotub, so any and all insights would be greatly appreciated. This is good. Sure it wont get you as many but hey, lil is better than none. *with the sole exception of remedies that do not involve breaching the sterile environment within the jar or involve incubation temps over 78(F). Monotub making pic 4 May 22, 2009 · Spawn runs shouldn't even be taking so long that any GE needs to take place. Otherwise contams will get ya. Sep 18, 2023 · Here is a step-by-step guide to the monotub method: 1. One of the most common reasons why monotubs stall is because there isn’t enough airflow. 124K subscribers in the shroomers community. When the substrate is fully colonized, evidenced by a white, mycelium-covered surface, it's time to encourage fruiting. Barley straw. The concern is that if pinning doesn't start soon, then contams would be more likely to gain a foothold. The tubs have a seal on the lid. First off, you'll need to get your mushroom grain spawn colonizing so it can be used in your monotub. Fanning should be performed for approximately 30 seconds, twice per day. my ratio was very low so I figured it would take long. The imperfect lids on these tubs is all you need for proper GE. Wipe down the lid (or a second tub turned upside down) and cover the monotub. After fanning, put the lid back on the monotub. May 25, 2010 · Gravity does that. So I finally got to the stages where I could take my spawn tub to bulk substrate in a MonoTub! I used CVG substrate, following GPT on YouTube. Some say to keep it in dark for ~2 weeks. For extra protection against contaminants, spray a paper towel with alcohol and wipe down the inside of your monotub above the liner and substrate. 14:58. Reply. Indirect light will help colonization times slightly. Hi friends of the mushroom. Should I keep a lid on the monotub without any fresh air exchange until it is in fruiting conditions. A monotub is a modified plastic tub, like those ones you see at big box stores, typically used for storing or moving items. After some reading I decided to shake them up, It seemed like the right thing to do since the Let your tubs colonize the surface 75% before introducing fruiting conditions. I just checked it and its about 60% colonized. Monotub making pic 2; On the ends make one mark ⅔ of the way up in the middle of the end. Quote: jingus said: if the substrate has been colonizing (hpoo/straw) for a week or so, and is 100% colonized, then you put a casing layer on it. Some say it's outdated advice and you should just keep it in normal day/light cycle like you're going to have once it's ready to fruit. Enoki. High humidity levels can lead Apr 9, 2014 · Mushrooms like mammals have a circadian rhythm. it definitely could have some bacterial contaminment it's been colonizing for way too long I don't think it necessarily does though I think it's just way colonized in spots and I did not break it up and mix very much as I should have (was trying to be way too gentle 🤦🏼‍♂️) I will upload some more pics of it out of the bag soon. about 10 days to pins . Jan 24, 2022 · The idea behind a monotub is to trap the humidity inside a confined space so it can build up to levels that are conducive to mushroom growth, while providing passive air exchange (Diffusion) of CO2 and Oxygen. where other cubes take 5 to 6 days to pins. Most people just use a non-breathable tape. The substrate should be sterilized to remove any Some common forms of straw used for a bulk substrate recipe while growing mushrooms are: Wheat straw. hey everyone i have a few tubs going now. The holes drilled long the side of the mono tub provide areas of passive air exchange and help retain water or humidity. Dec 25, 2012 · Cut your liner to shape and place it in the tub. I transferred spawn to the coir/Verm bulk sub 16 days ago. Apr 22, 2017 · Okay thanks buddy I've only got those 3x 10 W Led lights so far, and 1x 24 w CFL in at the ceiling, its like 50 cm from the monotubs, but i cant move it Okay, so i just put polyfill in, and turned on the lights, so i guess its in fruiting conditions now and the other the 3rd one still looks like it could use a little more colonization before its ready. Give them the right conditions and ignore them for days at a time. Step 3 : Cover Holes with Gorilla or Duct Tape on both sides. How to Inoculate a Spawn Bag with Agar. Opening the top of the bag to put agar in gives contams a chance to get in there and create havoc. Im not sure whats wrong. Expose some mycelium and be patient. Allow the mycelium to well recolonize the bag. But you can go lower to 1:3 or 1:4 even. I recommend lightly misting until it looks like these pics (don't overdo it), closing the lid, and just leaving it alone for a week. Fresh air is the number one pinning trigger. It's not showing any signs of mycelium growth, I could include a picture but it would literally just look like a tub of dirt. At that point, remove the tape and position the monotub in ambient light. Nov 16, 2012 · So what what precautions? If you colonize in a spawn bag, break up the substrate at 50% to 70%. I started this tub near the beginning of October. Sep 8, 2023 · Your monotub grow room should maintain an optimum temperature range between 75°F - 81°F (24°C - 27°C). Step 4: After you have introduced your monotub to fruiting conditions and a scheduled light cycle, you will see primordia start to form in Monotubs really do embody the three “E’s” you saw in the title of this article: easy, efficient, and effective. Follow the kit's instructions for the depth and distribution of the substrate. Apr 16, 2011 · Quote: highc said: Quote: Ajaxx said: Quote: MasterP1247 said: Mycelium in my monotub has been growing VERY slowly over a three week period. Common substrates used in the monotub method include a mixture of straw, sawdust, and other organic materials. Have a fish water heater in the bottom tub filled with water. Hey hey, been growing for awhile now and always get good results aside from god damn it takes me so fucking long lol. Your tub looks like it's about to blow up, but now your messing with it and removing the liner and potentially exposing it to contam unnecessary. When I opened my bucket in the morning after 24 hours of pasteurizing the substrate, it was very VERY over hydrated. Yeah looks fine,you shouldn't open it intell its fully colonized tho Feb 23, 2024 · Substrate Layering:Spread a layer of substrate provided in your kit at the bottom of the tub. Mushroom, Mycelium, Petri Dish Time-Lapse Compilation →. So, 1/3rd of the total volume should be grain. Let your tubs colonize first, like this. You already know this I’m sure but cover those holes so the tub can hold it’s humidity and it’s will make a barrier so trich spores can’t get in or contamination. It will encourage pinning from the top of the substrate rather than the edge or bottom. Newb question. On the long sides the tub make two marks about ⅔ of the way up the side wall, try to spread the holes equally apart. When I squeezed my substrate, lots of water was pouring out. Not enough humidity on the walls of the tub. Video Tutorial : Building a Monotub for Growing Mushrooms. Polyfil promotes FAE, not GE, if you don't want to tape it with duct tape or what have you, at the very least use micropore. Might be easier for a beginner to use a lower ratio. This spawn will colonize the substrate and eventually grow into mature mushrooms. A lot of people put it straight to fruiting conditions after spawning so you can do so whenever you want it’ll just probably be better the further you let it colonize even though I’ve seen a lot of people say they can’t tell a difference when colonizing and not colonizing Feb 25, 2010 · Ill give ya some info, I already know the problem, just need help on correcting it. Dunno about popcorn, but my monos colonize in 5 - 7 days at a 1:2 spawn ratio. Shiitake. Based on the dimensions listed online, you need between 16-20qts total including spawn and bulk substrate to reach an acceptable height in a tub that size. (this is sanitization). It's awkward that the British, who live on the opposite side of the planet, were the first to colonize Australia after the initial human arrival. In the context of mushroom cultivation, a monotub is used Mar 17, 2011 · The grain spawn colonized in a normal time period (16 days). Nov 16, 2014 · So this is the third monotub I've done, but this is the first time I've had slower colonization rates (due to colder temperatures). It’s day 4 and all the colonization is happening under the top layer am I doing something wrong. Cut open the top of the bag and either roll the bag on itself leaving ~1-2 inches. For this purpose, you can use microporous tape, a syringe port or cotton filters. Actives. After you place your block on top of the foil after removing it from the grow bag you want to get your spray bottle and spray the sides of your tub so it drips down the walls and then mist your block and put the lid back on. Quick Reply. Some people say you can just move it right into fruiting and I’ve heard others say that you should wait 10 days. Step 1: Make or buy mushroom grain spawn. ← How to grow mushrooms in a monotub. Everyday your want to fan out your tub 2-3 times a day for air exchange and mist it. Jan 7, 2016 · I have a monotub growing. I’m leaning towards the 10 days side and if that’s the case. Without more specific information, it's challenging to pinpoint the exact problem, but here are some common factors that can lead to a squishy monotub: High Humidity: Excessive moisture in your monotub can make it feel squishy. However, your holes are all sorts of wrong. A fully colonized monotub means that your substrate has been completely overrun by mycelium, which forms the basis for mushroom fruiting. Pinheads or primordia are knots of mycelium that proceed their development into small mushrooms. 5-3 inches from the bottom of the MonoTub. But that's the absolute most you should do, which will give the best results. Keep the Monotub in a warm and humid area, and mist it periodically to keep the humidity levels high. Consolidate: Some extra days for the mycelial network to strengthen and become thicker and more rizomorphic(sp). Not Enough Airflow. Monotub making pic 3; Drill out all 6 of your marks with the 1 ¼” hole saw bit. 4 Holes on the sides are taped and 2 holes are Nov 12, 2007 · The surest way to turn a slow to colonize spawn into a contaminated spawn is by trying to amend some perceived mistake midway through the colonization. For the bottom, holes place the drill about 2. Bulk casing mix is a combination of coco coir, vermiculite, lime, gypsum, and some other ingredients. I'd say its about 45% colonized at this point. So I have two Penis Envy tubs I started on 12/19/21 and 12/21/21 - each tub is a 650qt cooked coir brick and 7 qts of grain spawn (organic rye berries). Building a DIY Monotub for Growing Mushrooms. Buckets don't need much. May 25, 2011 · The factors affecting our colonization time include, but are not limited to, spawn ratio and health, ambient temperatures, GE/FAE, substrate ingredients, moisture content, and light. Jun 3, 2022 · There are several reasons why your monotub may be stalling. Before entering fruiting STEP 3: Blocking the Light & Lining the Monotub. This is a long post so TL;DR: Innoculated 11 bags with 0. Contamination Comic. and just so you know, my casing got trich but it didnt show up till the 12th day, dont know why,,, iv done this before and at least have done casing's not everyone reads all the books,maybe we should i like Leave it alone! One of the hardest and best lessons to learn is patience, and hands off. So I have 5 32oz jars fully colonized with GT for set-up in a 56 quart monotub. Colonize a few more jars or plan to do a smaller tub or something with trays with the jar you have. Four holes on each side of the monotub stuffed with polyfill. Monitor for pinning: Pinning is the growth of primordia, the mushrooms’ first visible stage of growth. but i think i might have cobweb now. ----- Oct 27, 2011 · It takes more than 10 days from bulk for pinning. Here are some of the most common: 01. Done that last Wednesday night. If you colonize in a spawn bag, do not open it to lay the trays the day it appears fully colonized. Whenever I google people seem to say bulksub takes like 5 days to 2 weeks to fully colonize but for me its more like 2 to 3 months. This tape should remain in place until you reach the final stage of mushroom cultivation. Don't introduce a tub that isn't 100% colonized into fruiting, as you'll probably end up with a contam. The warmer it is, the more likely any latent Yeah, so basically: bleach your shower, run your bath water until it’s lukewarm (think bathing a newborn warm), cup your hands below the faucet to create a waterfall into the monotub, and fill it up until the cake is submerged. I've had one monotub before and it took about a month for it to start fruiting and I started a second one about a month ago. Nov 20, 2015 · I ain't happy, Feeling bad. no experience with PE and PF cakes. Make sure to use extra so you can mix your substrate up without spilling any. Follow your tek to a tee and rule out what issue your monotub needs to address. Gather all your items: Tub, Drill, and Drill piece. Jan 2, 2012 · Past 3 weeks, I'd start to get a little worried. I always notice it condenses and pulls away from the Sep 6, 2020 · I'll try to link one at a time. In this video, I will show you how to grow mushrooms from start to finish in a monotub like the Boomer Shroomer Inflatable Monotub. Filtration. I keep a desklamp with a 6500K bulb on a 12/12 timer in my colonization room at all Alright yall, ive searched and searched for hours without any luck of finding an answer, and with shroomery down it doesnt make this any easier. This is the only reason why monotub isn't recommended for newbie growers. Apr 20, 2017 · Okay, so now its been a few days. If monotub contaminated, all the amount of mushrooms that could have been given from each small mushroom cake separately will be lost. That small patch of white has not grown at all in three days. All FAE occurs through tightly stuffed polyfil (two holes high, four holes low). 10 In medieval times, the Arabs extensively explored the Indian Ocean, but they never discovered Australia. 15:38. So about a month ago I innoculated these bags, shooting the spores straight into the center of the bag. Sterilization in a PC or something might melt it. How fast depends on how thoroughly you mix your bags. Inflatable Monotub Time-lapse #3. I've never seen anyone sterilize their bulk substrate, so sterilizing the bucket it goes in doesn't make sense. This environment encourages mycelium—the vegetative part of fungus—to colonize fully. But what does contamination look like? Often, you’ll notice unusual colors – think greens, blacks, and yellows – or off-putting smells. To the original poster, take the polyfill out at full colonization. I bought the substrate and casing mix from an online vendor. Fresh Air Exchange: As your tub begins to pin, you’ll want to increase the FAE. You can see my casing layer is having mycelium colonize through. If not it will begin with side pinning so take a peek between the sub and the side of the tub wall. So, during colonization phase, it is better to have a system that allows gas exchange. The top of the substrate is fully colonized, but there are still spots at the bottom that have not colonized (maybe too much water). Jan 27, 2012 · slow monotub colonization 14 days. Why is it taking so long to colonize the top of my casing. Now put the lid on the monotub and gently store it somewhere it wont be bumpted into. Yours is wet so fan away and no more misting!! Keep fanning three times a day, four hours apart, and give your tub some light every day and it will blossom. I checked them the other day and there was a tiny bit of myc in the corner. Primarily concerned with psilocybin containing mushrooms, but all psychoactive species are welcome. Grain spawn taking forevverrrr to colonize. Increase light exposure and allow more air circulation, as these conditions signal the mushrooms to start fruiting. I'm reading some conflicting advice on how to colonize a monotub once it's prepared with grain spawn. Whatever the real reason is, it means the mycelium is not happy. Feb 12, 2021 · Put your fruiting container/block inside the monotube on top of the perlite. Blueing is a sign of stress. Otherwise, leave it in ! Jun 24, 2012 · So, from this I infer that when a jar is "100% colonized" on the outside surface, it is also colonized on the inside. I just started my monotub 10 days ago I was wondering what could be the resone it’s barley colonized I have the holes tightly packed wit pollyfill an… Oct 27, 2011 · It takes more than 10 days from bulk for pinning. If you mix the time to full colonization is faster. Oat straw. Jus my mentality hah ----- Jan 29, 2011 · true and you are correct and i am sorry, but that does not change the fact that a casing that size should have been fully colonized by now. Sep 19, 2023 · Place this material in a container or tray within or near the monotub. Optimal temp depends on the species you are growing, generally between 24-27°C ;) 13. To encourage fruiting, subject the fully colonized monotub to 24 hours of indirect light exposure, a temperature ranging from 60°F to 80°F depending on the mushroom species, high humidity, fresh air exchange, and a low CO2 level. there are spots of solid white growth and then thin gray spots in between. This will be hard to see since your tub is not clear. . Colonized substrate contaminates less easily. With the right materials and preparation, you can create an environment that will help your mushrooms thrive. Mar 8, 2023 · Cover the top securely with foil and wait until colonization occurs, which usually takes between 7-14 days depending on conditions. Yes. After three weeks a small amount (%10) of the bag was colonized but is was spreading very slowly. It's the equivalent of seeds for mushroom growers. As it draws in moisture, it'll reduce the overall humidity. So, the short answer to this question is, yes, the grain spawn needs very little air exchange for optimal growth. . The change from the vegetative growth (mycelium growth or colonisation period) to the fruiting period (knots, pinheads and mushroom fruit bodies development) is called knotting, pinning, pin setting or pinhead initiation period. Sometimes it’ll float but that’s okay. To get them to colonize like this, let them sit undisturbed for a few days with the lid on. For the hunting, growing, and consuming of mushrooms. 8 weeks from innoculation to harvest. You want direct/intense 6500K light on a 12/12 schedule for fruiting. Mine looked like this a couple days ago and I've got pins starting yesterday. If we only top spawn, then the mycelium has to grow through 22cm of the substrate (30cm x 3/4). Let it colonize: Once the mushroom spawn has been added, it’s time to wait for the substrate to colonize. I have 15lbs of substrate casing mix A place to discuss the growing, hunting, and the experience of magical fungi. 5cc b+ each, spawned to bulk with a 650g coco coir brick in an unmodified monotub, got 2204g wet (151g dry) 1st flush. I followed Damiens 5050 tek and I have two monotubs, each with 5 quarts of fully colonized wbs. Monotubs need a lot of airflow to stay healthy, and if there isn’t enough, they can quickly stall. Place water in the foil container and cover with the mushroom block with the plastic bag. You'll be able to trim it after spawning. Good luck 👍 [general] just wanted to show off my inoculation box. You want evaporation on the surface so you mist and then fan but only mist of the surface isn't wet. I got monotub In a bag. You want ambient/indirect light (on a 12/12 schedule preferably) for colonization and consolidation. If you see or smell anything suspicious, it’s likely your monotub has fallen victim to contamination. So maybe it will take 8 days and maybe it will take 20 days, but when the substrate is ready, we will know if we just watch & listen to it. Aug 28, 2023 · Why is this so important? Mushrooms consume oxygen and release carbon dioxide, much like we humans do. The substrate jars are comprised of rye berries, milo, and millet. I think my monotub might have been a little bit on the dry side, so i put in some extra polyfill, and misted quite a bit but here's the results so far Im thinking about harvesting the biggest ones first, and let the smaller ones fruit a few more days, and then harvest everything, when the smaller ones mature How long have they been colonizing for? Do you have an opinion as to why the colonizing stalled? Is the colonization all on one part or is it diffuse? 18 votes, 26 comments. Wiping it down with alcohol is ok. Grain spawn is sterilized grain that has been fully colonized with mushroom mycelium. At a growth rate of 6 to 7 mm/day, it will take roughly 31-37 days to colonize the bag entirely. Fruiting took longer . Mushrooms grow towards light because they're deriving energy from it. Then attach the Drill piece to the head end of the drill. The grain is fully colonized first then mixed with the substrate. Give it between two and four more days. Common mushrooms species grown on straw substrate are: Oyster / King Oyster. Inoculation:Evenly distribute the spawn over the substrate. Reply reply. So long as the substrate was not over wet or compacted, etc. I didn't keep it at proper temperature so it had some 60F nights. Cutting an x in the front or top of a fruiting block works well for exposing mycelium. Tubs aren't air tight so enough FAE will get in through the lid still. Oct 23, 2008 · Longtime reader, this is my first post. For spawn to bulk you mix a few spawn jars or spawn bags in one tub. Take your colonized spawn jars, check for visible problems and break each Best to do a 1:2 or 1:3 spawn rate. If it fails to colonize or gets contaminated (most likely) consider it a lesson and move on from there. One thing you said colonization in dark I've been doing it in ambient light and the 2 bags in more light are colonizing faster than the 2 bags that are in the darker area , so I'm going to keep colonize spawn bags in ambient light and then colonize my monotub in total darkness. Monotubs with polyfil don't really need a whole lot of extra FAE. Simply flip the lid upside-down and place it on top of the tub. Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable. Thanks. For the cultivation process, it's essential to place clear tape over the holes on the inside of the monotub. Small tubs don’t need a lot of air, so the air that exchanges through the gap of the unsealed lid is plenty. In a confined space, especially in a monotub grow kit, the air can quickly become saturated with CO2, which could lead to stunted growth, increased susceptibility to contamination, and other growth-related issues. Has not let me down. If not then get some coir and use that. This is weird, because they discovered and colonized other remote places like Mozambique and Java. Not enough Grain spawn bulk, temps below 68 degrees, over hydrated substrate, under hydrated substrate. Blocking light from reaching the bottom and sides of the bulk substrate is an important step. May 21, 2009 · Fan on in the room (NOT pointed directly at monotub) running on 15 monutes every hour, coolmist running 24/7, and there are lots of water droplets on the sides and roof of the monotub, and water beaded all over the myc. I applied a casing layer (Jiffy seed-starting mix, pasteurized for one hour 140F-160F in a gusseted filter bag) to the two tubs on 1/6/22 when I saw knotting/pins, but in the two A squishy monotub in mushroom cultivation can be indicative of a few potential issues. I started out with 4 of them and won't go back. Fruiting bodies from the edge and bottom of a substrate are very difficult to harvest. Step 2 : Heat Aluminium Can and Punch out Holes on sides of Tub. This is my first "full size" monotub. You could move to fruiting with that if you want to. Now I have been doing some reading Sep 19, 2023 · If it is packed too tightly it will inhibit the speed the spawn colonizes 12. Nameko (although more likely to contaminate than on hardwood). Two weeks ago, I used 12 fully colonized 1/2 pint jars (BRF cakes) as spawn to colonize my newly made 32 quart Monotub. The bulk substrate colonized in a normal time period (10 days). Temps should be 72-76*F. Jan 18, 2022 · Quick Reply. At that point just soak it for a couple hours. Mycelium at all stages of growth benefit from light. bv cd ho hj rf gz ck cq vc rj